• Technology
• Bioreactor technology
• Instructive Bone Grafts
• Publications

Publications

Bioreactor Technology & Stem cell Therapy

• Schop D, van Dijkhuizen-Radersma R, Borgart E, Janssen FW, Rozenmuller H, Prins HJ, de Bruijn JD. Expansion of human mesenchymal stromal cells on microcarriers: growth and metabolism. J Tissue Eng Regen Med. (2009)
• Janssen FW, van Dijkhuizen-Radersma R, van Oorschot A, Oostra J, de Bruijn JD, van Blitterswijk CA. Human tissue-engineered bone produced in clinically relevant amounts using a semi-automated perfusion bioreactor system: a preliminary study. J Tissue Eng Regen Med. (2009)
• Higueara G, Schop D, Janssen F, van Dijkhuizen-Radersma R, van Boxtel T, van Blitterswijk CA. Quantifying in vitro growth and metabolism kinetics of human mesenchymal stem cells using a mathematic model. Tissue Eng Part A.(2009) 15(9):2653-63
• Schop D, Janssen FW, van Rijn LD, Fernandes H, Bloem RM, de Bruijn JD, van Dijkhuizen-Radersma R. Growth, metabolism, and growth inhibitors of mesenchymal stem cells. Tissue Eng Part A. (2009) 15(8):1877-86.
• Schop D, Janssen FW, Borgart E, de Bruijn JD, van Dijkhuizen-Radersma R. Expansion of mesenchymal stem cells using a microcarrier-based cultivation system: growth and metabolism. J Tissue Eng Regen Med. (2008) 2(2-3):126-35.
• Both SK, van der Muijsenberg AJ, van Blitterswijk CA, de Boer J, de Bruijn JD. A rapid and efficient method for expansion of human mesenchymal stem cells. Tissue Eng. 2007 Jan;13(1):3-9.
• Kruyt MC, de Bruijn JD, Wilson CE, Oner FC, van Blitterswijk CA, Verbout AJ, Dhert WJ. Viable osteogenic cells are obligatory for tissue-engineered ectopic bone formation in goats. Tissue Eng. 2003 Apr;9(2):327-36.

Instructive Bone Grafts

• van Gaalen SM, de Bruijn JD, Wilson CE, van Blitterswijk CA, Verbout AJ, Alblas J, Dhert WJ. Relating cell proliferation to in vivo bone formation in porous Ca/P scaffolds. J Biomed Mater Res A. 2009 Feb 2
• Brittijn SA, Duivesteijn SJ, Belmamoune M, Bertens LF, Bitter W, de Bruijn JD, Champagne DL, Cuppen E, Flik G, Vandenbroucke-Grauls CM, Janssen RA, de Jong IM, de Kloet ER, Kros A, Meijer AH, Metz JR, van der Sar AM, Schaaf MJ, Schulte-Merker S, Spaink HP, Tak PP, Verbeek FJ, Vervoordeldonk MJ, Vonk FJ, Witte F, Yuan H, Richardson MK. Zebrafish development and regeneration: new tools for biomedical research.  Int J Dev Biol. 2009;53(5-6):835-50. Review
• de Ruijter A, Meijer G, de Bruijn JD, Koole R. Curi-Os bet-TCP as grafting material in cleft patients. J Cranio-Maxillof. Surg. Vol 36, Suppl 1, PS14 2008 .
• van Blitterswijk CA, Thomsen P, Hubbell J, Cancedda R, de Bruijn JD, Lindahl A, Sohier J and Williams DF (eds), Tissue Engineering, Academic Press, Elsevier, ISBN-13:978-0-12-370869-4; ISBN-10: 0-12-370869-9, 2008
• de Bruijn JD, Shankar K, Yuan H and Habibovic P. Osteoinduction and its evaluation, In: Bioceramics and their clinical applications, T Kokubo (Ed), Woodhead Publishing, CRC Press, pp 199-219 (2008)
• Meijer GJ, de Bruijn JD, Koole R, van Blitterswijk CA.Cell based bone tissue engineering in jaw defects. Biomaterials. 2008 Jul;29(1):3053-61.
• Kruyt MC, de Bruijn JD, Rouwkema J, van Blitterswijk CA, Oner C, Verbout AJ, Dhert WJ. Analysis of the dynamics of bone formation, effect of cell seeding density and potential of allogeneic cells in cell-based bone tissue engineering in goats. Tissue Eng Part A 14(6):1081-1088 (2008).
• Eniwumide JO, Yuan H, Cartmell SH, Meijer GJ, de Bruijn JD. Ectopic bone formation in bone marrow stem cell seeded calcium phosphate scaffolds as compared to autograft and (cell seeded) allograft. Eur Cell Mater. 2007 Aug 1;14:30-8; Discussion 39.
• Meijer GJ, de Bruijn JD, Koole R, van Blitterswijk CA. Cell-based bone tissue engineering. PLoS Med. 2007 Feb;4(2):e9. Review.
• Kruyt MC, Dhert WJ, Oner FC, van Blitterswijk CA, Verbout AJ, de Bruijn JD. Analysis of ectopic and orthotopic bone formation in cell-based tissue-engineered constructs in goats. Biomaterials. 2007 Apr;28(10):1798-805.
• Kruyt MC, Wilson CE, de Bruijn JD, van Blitterswijk CA, Oner CF, Verbout AJ, Dhert WJ. The effect of cell-based bone tissue engineering in a goat transverse process model. Biomaterials. 2006 Oct;27(29):5099-106.
• Yuan H, van Blitterswijk CA, de Groot K, de Bruijn JD. Cross-species comparison of ectopic bone formation in biphasic calcium phosphate (BCP) and hydroxyapatite (HA) scaffolds. Tissue Eng. 2006 Jun;12(6):1607-15.
• Yuan H, van Blitterswijk CA, de Groot K, de Bruijn JD. A comparison of bone formation in biphasic calcium phosphate (BCP) and hydroxyapatite (HA) implanted in muscle and bone of dogs at different time periods. J Biomed Mater Res A. 2006 Jul;78(1):139-47.
• Kruyt MC, Persson C, Johansson G, Dhert WJ, de Bruijn JD. Towards injectable cell-based tissue-engineered bone: the effect of different calcium phosphate microparticles and pre-culturing. Tissue Eng. 2006 Feb;12(2):309-17.
• Wilson CE, Kruyt MC, de Bruijn JD, van Blitterswijk CA, Oner FC, Verbout AJ, Dhert WJ. A new in vivo screening model for posterior spinal bone formation: comparison of ten calcium phosphate ceramic material treatments. Biomaterials. 2006 Jan;27(3):302-14.
• de Bruijn, Discussion: a 1-year study of osteoinduction in hydroxyapatite-derived biomaterials in an adult sheep model: Part II. Bioengineering implants to optimize bone replacement in reconstruction of cranial defects. Plast Reconstr Surg 114(5):1164-1165 (2004)
• Kruyt MC, Dhert WJ, Yuan H, Wilson CE, van Blitterswijk CA, Verbout AJ, de Bruijn JD. Bone tissue engineering in a critical size defect compared to ectopic implantations in the goat. J Orthop Res. 2004 May;22(3):544-51.
• Kruyt MC, Dhert WJ, Oner C, van Blitterswijk CA, Verbout AJ, de Bruijn JD. Optimization of bone-tissue engineering in goats. J Biomed Mater Res B Appl Biomater. 2004 May 15;69(2):113-20.
• Kruyt MC, Dhert WJ, Oner C, van Blitterswijk CA, Verbout AJ, de Bruijn JD. Osteogenicity of autologous bone transplants in the goat. Transplantation. 2004 Feb 27;77(4):504-9.
• Kruyt MC, de Bruijn JD, Yuan H, van Blitterswijk CA, Verbout AJ, Oner FC, Dhert WJ. Optimization of bone tissue engineering in goats: a peroperative seeding method using cryopreserved cells and localized bone formation in calcium phosphate scaffolds. Transplantation. 2004 Feb 15;77(3):359-65.
• Kruyt MC, van Gaalen SM, Oner FC, Verbout AJ, de Bruijn JD, Dhert WJ. Bone tissue engineering and spinal fusion: the potential of hybrid constructs by combining osteoprogenitor cells and scaffolds. Biomaterials. 2004 Apr;25(9):1463-73. Review.
• Liu Y, Hunziker EB, Layrolle P, De Bruijn JD, De Groot K. Bone morphogenetic protein 2 incorporated into biomimetic coatings retains its biological activity. Tissue Eng. 2004 Jan-Feb;10(1-2):101-8.
• de Bruijn JD, van Blitterswijk CA, de Groot K , Yuan H. Calcium phosphate ceramics for bone tissue engineering. In Proceedings of the 8th CCT on Bioceramics surfaces: behaviour in vitro and in vivo, 2003
• Fischer EM, Layrolle P, Van Blitterswijk CA, De Bruijn JD. Bone formation by mesenchymal progenitor cells cultured on dense and microporous hydroxyapatite particles. Tissue Eng. 2003 Dec;9(6):1179
• Yuan H, Van Den Doel M, Li S, Van Blitterswijk CA, De Groot K, De Bruijn JD. A comparison of the osteoinductive potential of two calcium phosphate ceramics implanted intramuscularly in goats. J Mater Sci Mater Med. 2002 Dec;13(12):1271-5.
• Yuan H, De Bruijn JD, Zhang X, Van Blitterswijk CA, De Groot K. Use of an osteoinductive biomaterial as a bone morphogenetic protein carrier. J Mater Sci Mater Med. 2001 Sep;12(9):761-6.
• Yuan H, Yang Z, de Bruijn JD, de Groot K, Zhang X. Material-dependent bone induction by calcium phosphate ceramics: a 2.5-year study in dog. Biomaterials 22(19):2617-2623 (2001)
• Yuan H, de Bruijn JD, Zhang X, van Blitterswijk CA, de Groot K. Bone induction by porous glass ceramic made from Bioglass (45S5). J Biomed Mater Res. 2001 May 1;58(3):270-6.
• Yuan H, De Bruijn JD, Li Y, Feng J, Yang Z, De Groot K, Zhang X. Bone formation induced by calcium phosphate ceramics in soft tissue of dogs: a comparative study between porous alpha-TCP and beta-TCP. J Mater Sci Mater Med. 2001 Jan;12(1):7-13.
  
• de Bruijn JD, Yuan H, Dekker R, Layrolle P, de Groot K, van Blitterswijk CA. Osteoinductive biomimetic calcium phospate coatings and their potential use as tissue engineering scaffolds. in Bone Engineering, JE Davies (Ed), EM Square Inc, Toronto, pp 421-431 (2000)
• Meijer GJ, de Bruijn JD, van Blitterswijk CA. Tissue engineering of bone, the future ?! Z Zahnarztl Implantol 16:207-211 (2000)
• Yuan H, Li Y, de Bruijn JD, de Groot K, Zhang X. Tissue responses of calcium phosphate cement: a study in dogs. Biomaterials. 2000 Jun;21(12):1283-90.
• Yuan H, Kurashina K, de Bruijn JD, Li Y, de Groot K, Zhang X. A preliminary study on osteoinduction of two kinds of calcium phosphate ceramics. Biomaterials. 1999 Oct;20(19):1799-806.
• Anderson ML, Dhert WJ, de Bruijn JD, Dalmeijer RA, Leenders H, van Blitterswijk CA, Verbout AJ. Critical size defect in the goat's os ilium. A model to evaluate bone grafts and substitutes. Clin Orthop Relat Res. 1999 Jul;(364):231-9.
• Yuan H, Yang Z, Li Y, Zhang X, De Bruijn JD, De Groot K. Osteoinduction by calcium phosphate biomaterials.  J Mater Sci Mater Med. 1998 Dec;9(12):723-6.